| 产品名称 |
Recombinant S. hygroscopicus BAR |
| 英文名称 |
Phosphinothricin N-acetyltransferase/Bar |
| 纯度 |
Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 |
<1 EU/µg as determined by LAL test. |
| 蛋白构建 |
Recombinant Streptomyces Hygroscopicus Phosphinothricin N-acetyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed. |
| Accession |
P16426 |
| 表达宿主 |
E.coli |
| 种属 |
Streptomyces hygroscopicus |
| 预测分子量 |
20.6 KDa |
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 12.5mM Tris-HCl, 50mM NaCl, /u200e/u200e5% Trehalose, 5% Mannitol, 0.01% Tween 80, 2mM DTT, 1mM EDTA, pH8.5. |
| 运输方式 |
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 |
Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 分子别名 |
| Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; Phosphinothricin-resistance protein; bar |
| 背景介绍 |
| Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications. |
注意事项
本司产品仅用于科研，不用于临床诊断和治疗
