| 产品名称 |
Recombinant Human TK1 (C-6His) |
| 英文名称 |
Thymidine kinase, cytosolic/TK1 |
| 纯度 |
Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 |
<1 EU/µg as determined by LAL test. |
| 蛋白构建 |
Recombinant Human Thymidine Kinase, Cytosolic is produced by our Mammalian expression system and the target gene encoding Met1-Asn234 is expressed with a 6His tag at the C-terminus. |
| Accession |
P04183 |
| 表达宿主 |
Human Cells |
| 种属 |
Human |
| 预测分子量 |
26.5 KDa |
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 5% Trehalose, 5% Mannitol, 500 mM NaCl, 2 mM EDTA, 10% Glycerol, 1 m MDTT, 0.05% Tween80, pH8.0. |
| 运输方式 |
The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 |
Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 |
| 分子别名 |
| Thymidine kinase; cytosolic; TK1 |
| 背景介绍 |
| Thymidine kinase 1(TK1) belongs to the thymidine kinase family. It is located in the cytoplasm, and phosphorylated on Ser-13 in mitosis during post-translational modification. Two forms of this protein have been identified in animal cells, one in cytosol TK1 and one in mitochondria TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, while it is very low in resting cells. TK1 acts as a homotetramer, and can transform thymidime to thymidine 5-phosphate with the help of ATP |
注意事项
本司产品仅用于科研，不用于临床诊断和治疗
