RNF20 Mouse Monoclonal Antibody-EnkiLife

RNF20 Mouse Monoclonal Antibody

规格：50μL 价格：￥1350
规格：100μL 价格：￥2250
应用（Application）：WB
种属（Reactivity）：Human, Mouse
偶联物（Conjugate）：Unconjugated
基因名（Gene Name）：RNF20 SKU: AMM86011 Category: 鼠单克隆抗体 Tags: Mouse, Unconjugated, WB, Human, Mouse

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产品概述

产品名称（Product Name）	RNF20 Mouse Monoclonal Antibody
描述（Description）	Mouse monoclonal Antibody
宿主（Host）	Mouse
应用（Application）	WB
种属反应性（Reactivity）	Human, Mouse

产品性能

偶联物（Conjugation）	Unconjugated
修饰（Modification）	Unmodified
同种型（Isotype）	Mouse IgG1
克隆（Clonality）	Monoclonal
形式（Form）	Liquid
存放说明（Storage）	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
储存溶液（Buffer）	Purified antibody in PBS with 0.05% sodium azide.
纯化方式（Purification）	Affinity Purification

免疫原

基因名（Gene Name）	RNF20
别名（Alternative Names）	E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, 632-, RING finger protein 20, RNF20, BRE1A
基因ID（Gene ID）	56254
蛋白ID（SwissProt ID）	Q5VTR2

产品应用

稀释比（Dilution Ratio）	WB 1:1000-1:2000
蛋白分子量（Molecular Weight）	113.7kDa

研究背景

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

研究领域