| 产品名称 |
Recombinant Human MGMT (N-6His) |
| 英文名称 |
Methylated-DNA--protein-cysteine methyltransferase /MGMT |
| 纯度 |
Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 |
<1 EU/µg as determined by LAL test. |
| 蛋白构建 |
Recombinant Human Methylated-DNA-protein-cysteine Methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus. |
| Accession |
P16455 |
| 表达宿主 |
E.coli |
| 种属 |
Human |
| 预测分子量 |
23.8 KDa |
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl,1mM DTT,1mM EDTA,500mM NaCl,0.1%Trition X-100,pH 8.0. |
| 运输方式 |
The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 |
Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 |
| 分子别名 |
| Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNAmethyltransferase;O-6-methylguanine-DNA-alkyltransferase; MGMT |
| 背景介绍 |
| MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine. |
注意事项
本司产品仅用于科研，不用于临床诊断和治疗
