4E-BP1/2/3 (Phospho-Thr 45) Mouse Monoclonal Antibody-EnkiLife

4E-BP1/2/3 (Phospho-Thr 45) Mouse Monoclonal Antibody

规格：50μL 价格：￥1350
规格：100μL 价格：￥2250
应用（Application）：WB,IP
种属（Reactivity）：Human, Mouse, Rat
偶联物（Conjugate）：Unconjugated
基因名（Gene Name）：4E-BP1/2/3 (Phospho-Thr 45) SKU: AMM86144 Category: 鼠单克隆抗体 Tags: Mouse, Unconjugated, WB, IP, Human, Mouse, Rat

说明书复制

产品概述

产品名称（Product Name）	4E-BP1/2/3 (Phospho-Thr 45) Mouse Monoclonal Antibody
描述（Description）	Mouse monoclonal Antibody
宿主（Host）	Mouse
应用（Application）	WB,IP
种属反应性（Reactivity）	Human, Mouse, Rat

产品性能

偶联物（Conjugation）	Unconjugated
修饰（Modification）	Phosphorylated
同种型（Isotype）	IgG
克隆（Clonality）	Monoclonal
形式（Form）	Liquid
存放说明（Storage）	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
储存溶液（Buffer）	Purified antibody in PBS with 0.05% sodium azide and 50% glycerol.
纯化方式（Purification）	Affinity Purification

免疫原

基因名（Gene Name）	4E-BP1/2/3 (Phospho-Thr 45)
别名（Alternative Names）	4E-BP1 antibody 4EBP1 antibody 4EBP1_HUMAN antibody BP 1 antibody eIF4E binding protein 1 antibody eIF4E-binding protein 1 antibody Eif4ebp1 antibody Eukaryotic translation initiation factor 4E-binding protein 1 antibody PHAS-I antibody PHASI antibody Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
基因ID（Gene ID）	1978
蛋白ID（SwissProt ID）	Q13541

产品应用

稀释比（Dilution Ratio）	WB 1:500-1:2000,IP 1:20-1:50
蛋白分子量（Molecular Weight）	21kDa

研究背景

The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.

研究领域