| 产品名称 |
Recombinant Human TDT (N-6His) |
| 英文名称 |
DNTT/TDT |
| 纯度 |
Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 |
<1 EU/µg as determined by LAL test. |
| 蛋白构建 |
Recombinant Human DNA Nucleotidylexotransferase is produced by our E.coli expression system and the target gene encoding Met1-Ala509 is expressed with a 6His tag at the N-terminus. |
| Accession |
P04053 |
| 表达宿主 |
E.coli |
| 种属 |
Human |
| 预测分子量 |
60.7 KDa |
| 制剂 |
Supplied as a 0.2 μm filtered solution of 0.1M KH2POsub//u003E4/sub//u003E, 200mM KCl, 1mM 2-Mercaptoethanol, 50% Glycerol, pH 7.2. |
| 运输方式 |
The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 |
Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 |
| 分子别名 |
| DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltr; Terminal deoxynucleotidyltransferase; Terminal transferase; DNTT; TDT |
| 背景介绍 |
| Terminal deoxynucleotidyl transferase (TdT) is a highly conserved vertebrate enzyme that possesses the unique ability to catalyze the random addition of deoxynucleoside 5-triphosphates onto the 3-hydroxyl group of a single-stranded DNA. It plays an important role in the generation of immunoglobin and T-cell receptor diversity. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. |
注意事项
本司产品仅用于科研，不用于临床诊断和治疗
