| 产品名称 |
Recombinant Human EIF4E |
| 英文名称 |
eIF-4E/Eukaryotic translation initiation factor 4E |
| 纯度 |
Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 |
<1 EU/µg as determined by LAL test. |
| 蛋白构建 |
Recombinant Human Eukaryotic Translation Initiation Factor 4E is produced by our E.coli expression system and the target gene encoding Met1-Val217 is expressed. |
| Accession |
AAH12611.1 |
| 表达宿主 |
E.coli |
| 种属 |
Human |
| 预测分子量 |
25.1 KDa |
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 6% Trehalose, 0.05% Tween80, pH8.0. |
| 运输方式 |
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 |
Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 分子别名 |
| Eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; EIF4E; EIF4EL1; EIF4F |
| 背景介绍 |
| Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. |
注意事项
本司产品仅用于科研,不用于临床诊断和治疗