| 产品名称 |
Recombinant Human KPNB1 (N-6His) |
| 英文名称 |
Importin Subunit Beta-1/KPNB1 |
| 纯度 |
Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 |
<1 EU/µg as determined by LAL test. |
| 蛋白构建 |
Recombinant Human Importin Subunit Beta-1 is produced by our E.coli expression system and the target gene encoding Met1-Ala876 (Gln559His) is expressed with a 6His tag at the N-terminus. |
| Accession |
Q14974 |
| 表达宿主 |
E.coli |
| 种属 |
Human |
| 预测分子量 |
98.6 KDa |
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, 30% Glycerol, 100mM Nacl, pH 8.0. |
| 运输方式 |
The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 |
Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 |
| 分子别名 |
| Importin subunit beta-1; Importin-90; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97; KPNB1; NTF97 |
| 背景介绍 |
| Importin subunit beta-1(KPNB1) is a member of the importin beta family. KPNB1 contains 1 importin N-terminal domain and 19 HEAT repeats. It is involved in nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. Its functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. The import of proteins containing a classical nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits. Each of these subunits is part of the karyopherin family of proteins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. It mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. |
注意事项
本司产品仅用于科研，不用于临床诊断和治疗
