| 产品名称 |
Recombinant Carassiusauratus Leptin (N-8His) |
| 英文名称 |
Leptin |
| 纯度 |
Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 |
<1 EU/µg as determined by LAL test. |
| 蛋白构建 |
Recombinant Carassiusauratus Leptin is produced by our Yeast expression system and the target gene encoding Pro22-Cys171 is expressed with a 8His tag at the N-terminus. |
| Accession |
B8YI02 |
| 表达宿主 |
Yeast |
| 种属 |
Carassius auratus |
| 预测分子量 |
18.3 KDa |
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Citrate, 8% Trehalose, 4% Mannitol, 0.02% Tween80 (w/v), pH 5.5. |
| 运输方式 |
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 分子别名 |
| Leptin; Obese Protein; Obesity Factor; LEP; OB; OBS |
| 背景介绍 |
| Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. In mammals, leptin is mainly produced by the adipose tissue and encodes body fat reserves, acting as a short-term satiety signal. In fish, the presence of a leptin-like peptide was first evidenced by immuno-cross-reactivity, and its existence was certainly demonstrated after the finding by synteny of a leptin sequence in the pufferfish. |
注意事项
本司产品仅用于科研，不用于临床诊断和治疗
