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RNF20 (12E8) Rabbit Monoclonal Antibody【50ul/100ul买二送一】

RNF20 (12E8) Rabbit Monoclonal Antibody【50ul/100ul买二送一】

Cat: AMRe17291
★ ★ ★ ★ ★
规格：50μL 价格：￥1350
规格：100μL 价格：￥2250
应用（Application）：WB,ICC/IF,FC,IP
种属（Reactivity）：Human,Mouse
偶联物（Conjugate）：Unconjugated
基因名（Gene Name）：RNF20
分类: 兔单克隆抗体 标签: , , , , , , , ,

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产品概述

产品名称（Product Name）

RNF20 (12E8) Rabbit Monoclonal Antibody

描述（Description）

Recombinant rabbit monoclonal antibody

宿主（Host）

Rabbit

应用（Application）

WB,ICC/IF,FC,IP

种属反应性（Reactivity）

Human,Mouse

 

产品性能

偶联物（Conjugation）

Unconjugated

修饰（Modification）

Unmodified

同种型（Isotype）

IgG

克隆（Clonality）

Monoclonal

形式（Form）

Liquid

存放说明（Storage）

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

储存溶液（Buffer）

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

纯化方式（Purification）

Affinity purification

 

免疫原

基因名（Gene Name）

RNF20

别名（Alternative Names）

BRE1-A; hBRE1; RING finger protein 20; E3 ubiquitin-protein ligase BRE1A; RNF20;

基因ID（Gene ID）

56254

蛋白ID（SwissProt ID）

Q5VTR2

 

产品应用

稀释比（Dilution Ratio）

WB 1:500-1:2000,ICC/IF 1:100-1:200,FC 1:100-1:200,IP 1:50-1:100

蛋白分子量（Molecular Weight）

114kDa

 

研究背景

RNF20 is an E3 ubiquitin ligase protein that mediates monoubiquitination of histone H2B and the methylation of histone H3. It forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. It is required for transcriptional activation of Hox genes. Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

 

研究领域

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