| 产品货号 | APRab80033 |
| 产品名称 | GFP/eGFP Tag Rabbit polyclonal Antibody |
| 推荐应用 | WB,IHC-P,ICC/IF,FC |
| 反应种属 | Species-independent |
| 纯度 | Affinity Chromatography |
| 基因名称 | eGFP |
| 免疫原 | Purified recombinant fragment expressed in E. Coli. |
| 特异性 | The antibody detects GFP-tag fusion proteins. |
| 稀释度 | WB-1:1000-1:5000; IHC:1:100-1:200; ICC/IF-1:200-1:500; FC-1:20-1:50 |
| 宿主 | Rabbit |
| 同种型 | IgG |
| 存储缓冲液 | Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| 运输及保存 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景介绍 | C8CHS1.Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization. |
