The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
基因名称
GFP
免疫原
Purified recombinant fragment of GFP expressed in E. Coli.
特异性
The antibody detects GFP-tag fusion proteins.
稀释度
WB 1:5000, ELISA 1:5000-20000, Optimal dilutions should be determined by the end user
宿主
Mouse
同种型
IgG1
存储缓冲液
Liquid in PBS containing 50% glycerol, 2% BSA and New type preservative N.
运输及保存
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
背景介绍
GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
