The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
基因名称
His
免疫原
Synthetic peptide:HHHHHH conjugated to KLH.
特异性
The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.
稀释度
WB 1:5000, ELISA 1:5000-20000, Optimal dilutions should be determined by the end user
宿主
Rabbit
同种型
IgG
存储缓冲液
Liquid in PBS containing 50% glycerol, 2% BSA and New type preservative N.
运输及保存
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
背景介绍
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
